Study on the orientation of pigment in thylakoid based on polarization technique

The absorption spectrum and fluorescence spectrum of thylakoid(sample I) and chlorophyll(sample II) extracted from brassica chinensis were studied based on polarization technique. As a result, the absorption peak positions of sample I red shift by more than ten nanometers comparing sample II and the absorption intensities of sample I declined when the polarizer went round from 0°to 90°. It gave detailed explanation why the two absorption spectra feel so different. On the other side, the polarization fluorescence spectra of the two samples excited by 437nm were investigated respectively and the calculation of the fluorescence polarization degree which is determined by the environment of the pigment, the bound state, the effectual energy transfer and the ordered arrangement of the pigment in the two samples showed that the pigment in sample I arrayed regularily .The results of the study would provide powerful reference to the research of the energy transfer and transformation during photosynthesis.