Title :
Vibrational photoacoustic microscopy for depth-resolved bond-selective imaging of tissues and organisms
Author :
Wang, Han-Wei ; Chai, Ning ; Hu, Song ; Dou, Wei ; Umulis, David ; Wang, Lihong V. ; Sturek, Michael ; Lucht, Robert ; Cheng, Ji-Xin
Author_Institution :
Weldon Sch. of Biomed. Eng., Purdue Univ., West Lafayette, IN, USA
Abstract :
We realize vibrational photoacoustic microscopy (VPA) using molecular excitation of overtone vibration and acoustic detection of the resultant pressure transients. We demonstrate 3-D VPA imaging of lipid-rich atherosclerotic plaques and lipid bodies in living Drosophila larvae by exciting 2nd overtone of the CH bond stretch around 8300 cm-1. Depth-resolved spectral analysis and a penetration depth of several mm are available.
Keywords :
bioacoustics; biological tissues; biomedical optical imaging; photoacoustic effect; 3D VPA imaging; Drosophila larvae; biological tissues; bond stretch; depth-resolved bond-selective imaging; lipid-rich atherosclerotic plaque; molecular excitation; organisms; vibrational photoacoustic microscopy; Absorption; Atherosclerosis; Biology; Lipidomics; Microscopy; Vibrations;
Conference_Titel :
Lasers and Electro-Optics (CLEO), 2011 Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-4577-1223-4
