Title :
DNA melting analysis on a microchip after PCR amplification
Author :
Slyadnev, M.N. ; Sato, K. ; Tokeshi, M. ; Kitamori, T.
Author_Institution :
Kanagawa Acad. of Sci. & Technol., Kawasaki, Japan
fDate :
Oct. 31 2001-Nov. 2 2001
Abstract :
DNA melting analysis requires precise temperature measurement. Since heat capacity of the sample inside microchip is extremely small, a measurement device can affect temperature response. In this paper we report a method of temperature monitoring that was implemented on a chip to overcome this difficulties.
Keywords :
DNA; biosensors; biothermics; melting; microsensors; temperature sensors; DNA melting analysis; PCR amplification; microchip; temperature measurement; Chemical technology; Chemistry; DNA; Fluorescence; Laser excitation; Microchannel; Optical filters; Semiconductor device measurement; Temperature measurement; Temperature sensors;
Conference_Titel :
Microprocesses and Nanotechnology Conference, 2001 International
Conference_Location :
Shimane, Japan
Print_ISBN :
4-89114-017-8
DOI :
10.1109/IMNC.2001.984156
