Title :
Three-dimensional motion of a nanoparticle on the cell membrane observed by non-interferometric widefield optical profilometry
Author :
Lee, Chau-Hwang ; Wang, Chun-Chieh ; Lee, Chia-Wei ; Huang, Chia-Yun ; Lin, Jiunn-Yuan ; Wei, Pei-Kuen
Author_Institution :
Res. Center for Appl. Sci., Acad. Sinica, Taipei
Abstract :
We directly observe three-dimensional motion of a gold nanoparticle on the membrane of a living cell by using non-interferometric widefield optical profilometry. We identify the occurrence of particle internalization and actin aggregation which indicates endocytosis.
Keywords :
biomedical measurement; biomembranes; cellular biophysics; nanoparticles; nanotechnology; optical instruments; surface topography measurement; actin aggregation; cell membrane; endocytosis; gold nanoparticle; living cell; noninterferometric widefield optical profilometry; particle internalization; three-dimensional motion; Biomedical optical imaging; Biomembranes; Cells (biology); Fluorescence; Gold; Nanobioscience; Nanoparticles; Optical scattering; Particle scattering; Surfaces; (170.1530) Cell analysis; (170.3880) Medical and biological imaging;
Conference_Titel :
Lasers and Electro-Optics, 2008 and 2008 Conference on Quantum Electronics and Laser Science. CLEO/QELS 2008. Conference on
Conference_Location :
San Jose, CA
Print_ISBN :
978-1-55752-859-9
