Title :
Second harmonic generation microscopy on the polyhedral inclusion bodies of nuclear polyhedrosis viruses
Author :
Liu, Tzu-Ming ; Lee, Yen-Wei ; Chang, Chieh-Feng ; Yeh, Shih-Chia ; Wang, Chung-Hsiung ; Chu, Shi-Wei ; Sun, Chi-Kuang
Author_Institution :
Grad. Inst. of Photonics & Optoelectron., Nat. Taiwan Univ., Taipei
Abstract :
We demonstrate second-harmonic-generation (SHG) microscopic imaging on the polyhedral inclusion bodies (PIB) of nucleopolyhedrovirus in living cells. Due to a body-centeredcubic arrangement of polyhedrin trimers, these PIBs generate SHG with polarization anisotropy.
Keywords :
biological techniques; cellular biophysics; light polarisation; microorganisms; optical harmonic generation; optical microscopy; body-centered-cubic arrangement; living cells; microscopic imaging; nuclear polyhedrosis viruses; nucleopolyhedrovirus; polarization anisotropy; polyhedral inclusion bodies; polyhedrin trimers; second harmonic generation; Biomedical optical imaging; Frequency conversion; Nonlinear optics; Optical harmonic generation; Optical microscopy; Optical polarization; Optical pulses; Photonics; Ultrafast optics; Viruses (medical); (180.4315) Nonlinear microscopy; (190.3970) Microparticle nonlinear optics; (190.4710) Optical nonlinearities in organic materials;
Conference_Titel :
Lasers and Electro-Optics, 2008 and 2008 Conference on Quantum Electronics and Laser Science. CLEO/QELS 2008. Conference on
Conference_Location :
San Jose, CA
Print_ISBN :
978-1-55752-859-9
