DocumentCode :
2274436
Title :
Mass spectrometry of biological molecules in ices and gels enabled by resonant picosecond IR laser excitation
Author :
Baltz-Knorr, M.L. ; Schriver, K.E. ; Haglund, R.F., Jr. ; Ermer, D.R.
Author_Institution :
Dept. of Phys. & Astron., Vanderbilt Univ., Nashville, TN, USA
fYear :
2002
fDate :
24-24 May 2002
Abstract :
Summary form only given. In proteomics and molecular biology, there is a significant unmet need for identifying proteins and peptides in either their native aqueous environment or in the gel media used for diffusion based separation and sequencing of proteins and nucleotides. Here we report the first observations of desorption and ionization of peptides and proteins directly from water ices and electrophoresis gels, without any additional sample preparation. This procedure, if further developed, could open the way to direct, high-speed molecular imaging of proteins that have undergone preliminary separation in a gel. In our experiments, picosecond laser pulses from a tunable, mid-infrared free-electron laser (FEL) ablate microscopic volumes of gel or ice containing polypeptides and proteins from a cryogenically cooled probe stage in a reflectron time-of-flight mass spectrometer at a temperature low enough to be vacuum-compatible.
Keywords :
biological techniques; free electron lasers; gels; high-speed optical techniques; laser ablation; molecular biophysics; proteins; time of flight mass spectra; cryogenically cooled probe stage; diffusion based protein separation; gel media; high-speed molecular imaging; microscopic volumes; molecular biology; nucleotides; peptides; picosecond laser pulses; protein sequencing; proteins identification; proteomics; sample preparation; time-of-flight mass spectrometer; tunable mid-infrared free-electron laser; vacuum-compatible; Electrokinetics; Free electron lasers; Ice; Ionization; Laser excitation; Mass spectroscopy; Peptides; Proteins; Proteomics; Resonance;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Lasers and Electro-Optics, 2002. CLEO '02. Technical Digest. Summaries of Papers Presented at the
Conference_Location :
Long Beach, CA, USA
Print_ISBN :
1-55752-706-7
Type :
conf
DOI :
10.1109/CLEO.2002.1034316
Filename :
1034316
Link To Document :
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