Title :
SRS microscopy as a low-invasive 3D bioimaging technique
Author :
Itoh, Kazuyoshi ; Ozeki, Yasuyuki
Author_Institution :
Grad. Sch., Dept. of Mater. & Life Sci., Osaka Univ., Suita, Japan
Abstract :
We introduce stimulated Raman scattering microscopy which allows us to visualize unstained live cells with high sensitivity and high contrast. The shot-noise limited sensitivity can be achieved based on high-frequency lock-in detection using subharmonically synchronized two-color pulses.
Keywords :
Raman spectroscopy; biomedical optical imaging; cellular biophysics; optical microscopy; shot noise; high-frequency lock-in detection; image contrast; lowinvasive 3D bioimaging technique; shot-noise limited sensitivity; stimulated Eaman scattering microscopy; subharmonically synchronized two-color pulses; unstained live cells; Laser noise; Microscopy; Optical microscopy; Raman scattering; Sensitivity; Synchronization;
Conference_Titel :
Information Optics (WIO), 2010 9th Euro-American Workshop on
Conference_Location :
Helsinki
Print_ISBN :
978-1-4244-8226-9
Electronic_ISBN :
978-1-4244-8227-6
DOI :
10.1109/WIO.2010.5582519
