Injection and laser manipulation of nanotool using photo responsive chemical for intracellular measurement

We developed selective cell injection of nanotool using photo responsive chemical for intracellular measurement. The nanotool was included in the fusogenic liposome. Membrane fusion of the liposome to the cell membrane was used for invasive cell injection of the nanotool. The liposome fuses to the cell in weak acidic condition. Local pH control inside the liposome was developed by using photo responsive chemical included in the nanotool. The nanotool was modified by fluorescent dye for environment measurement. The nanotool was also modified by photo responsive chemical Leuco crystal violet (LCV). LCV emits the proton by UV illumination. The emitted proton decreases pH value inside the liposome. This pH control is reversible by UV/VIS illumination. The nanotool included the liposome was manipulated by optical tweezers. After contact of the liposome to the cell membrane, the liposome was adhered to the cell membrane by UV-ray. The nanotool was injected into the cell autonomously. Injected nanotool was manipulated by optical tweezers. Intracellular temperature was also detected by measuring the fluorescence intensity of the nanotool. We demonstrated photo-induced cell injection of the nanotool and manipulation of the nanotool inside the cell.