An electrical DNA sensor with gold decorated SWCNT network

Author

Ko, Jung Wook ; Ahn, Jin-Hong ; Cheon, Jun Ho ; Kang, Tae June ; Kim, Yong Hyup ; Park, Young June

Author_Institution

NSI-NCRC, Seoul Nat. Univ., Seoul, South Korea

fYear

2010

fDate

17-20 Aug. 2010

Firstpage

1064

Lastpage

1067

Abstract

We report a novel electrical DNA biosensor, which consists of gold (Au) nano islands and SWCNT net on top of a semi-concentric Au electrode array (also referred to as the CGi). The real time measurement in TE buffer solution in a wide range of the target ss-DNA concentration down to fM (10^-15 M) was performed to check the detection limit. The CGi, functionalized with 25mer probe ss-DNA (5´-HSC₆-C₁₈-GC CAT TCT CAC CGH ATT CAG TCG TC-3´) shows high binding affinity of complementary ss-DNA targets until 10^-13 M. Moreover, it differentiates the mismatched target ss-DNA with high selectivity. This kind of CGi sensor platform can be applied to a target specific biological recognition element such as aptamer and protein easily, thereby realizing a general sensor platform for sensing many different types of biomolecules.

Keywords

DNA; biomedical materials; biosensors; carbon nanotubes; electric sensing devices; electrodes; gold; macromolecules; molecular biophysics; nanostructured materials; proteins; Au-C; DNA concentration; SWCNT net; aptamer; biomolecules; buffer solution; detection limit; electrical DNA biosensor; gold nanoislands; protein; semiconcentric electrode array; sensor platform; specific biological recognition element;

fLanguage

English

Publisher

ieee

Conference_Titel

Nanotechnology (IEEE-NANO), 2010 10th IEEE Conference on

Conference_Location

Seoul

ISSN

1944-9399

Print_ISBN

978-1-4244-7033-4

Electronic_ISBN

1944-9399

Type

conf

DOI

10.1109/NANO.2010.5698029

Filename

5698029