High-density antibody-immobilized surface for adhesion-based cell separation

We develop a versatile biomolecule immobilization method using amino-functionalized parylene, and examine the surface density of antibody immobilized on the parylene surface by using quartz crystal microbalance (QCM). We also demonstrate label-free cell sorting for the extraction of rare cells from a limited amount of sample. The sorting principle is based on cell-type specific adhesion with the aid of high-density surface-immobilized antibodies. Continuous lateral separation is also successfully achieved with antibody-immobilized oblique micro grooves in a micro channel.