Title :
Template construction and matching for identification of cells in differential interference contrast microscope images
Author :
Young, D. ; Gray, A.J.
Author_Institution :
Dept. of Stat. & Modelling Sci., Strathclyde Univ., Glasgow, UK
Abstract :
Template matching, for identification of cells in differential interference contrast (DIC) microscope images by digital image analysis, is complex. This is due to the nature of DIC images, namely an apparent illumination effect across cells in the image. Different cell sizes and orientations cause further complications. Three methods of template construction are proposed for different types of cell, and a successful automatic method for cell counting and sizing is described. These methods compensate for the complexities in the images
Keywords :
biological techniques; biology computing; cellular biophysics; image processing; optical images; optical microscopy; apparent illumination effect; biological research technique; cell counting; cell sizing; cells identification; differential interference contrast microscope images; digital image analysis; image complexities compensation; successful automatic method; template construction; template matching; Algae; Biomass; Digital images; Image analysis; Interference; Lighting; Microorganisms; Optical microscopy; Shape; Statistics;
Conference_Titel :
Image Analysis and Interpretation, 1998 IEEE Southwest Symposium on
Conference_Location :
Tucson, AZ
Print_ISBN :
0-7803-4876-1
DOI :
10.1109/IAI.1998.666892
