Immobilized quantum dot bioprobes: Fiber optics and microfluidics for the development of nucleic acid assays

Author

Tavares, A.J. ; Noor, O. ; Vannoy, C. ; Chen, L. ; Algar, W.R. ; Krull, U.J.

Author_Institution

Dept. of Chem. & Phys. Sci., Univ. of Toronto Mississauga, Mississauga, ON, Canada

fYear

2011

fDate

18-20 May 2011

Firstpage

1

Lastpage

2

Abstract

Multiplexed fluorescence-based assays are possible by immobilization of mixtures of different nucleic acid QD-bioprobes onto fused silica optical fibers, and onto glass surfaces of glass-PDMS microfluidic chips. Dynamic control of stringency by adjustment of the electrical potential to drive electroosmotic flow is advantageous. QD-bioprobes can be covalently anchored, or can be immobilized using a labile tethering system for removal and replacement.

Keywords

II-VI semiconductors; bioMEMS; cadmium compounds; fluorescence; glass; macromolecules; microfluidics; mixtures; molecular biophysics; optical fibres; semiconductor quantum dots; silicon compounds; wide band gap semiconductors; zinc compounds; CdSe-ZnS; SiO₂; covalently anchoring; electroosmotic flow; fiber optics; fused silica optical fibers; glass surfaces; glass-PDMS microfluidic chips; immobilized quantum dot bioprobes; labile tethering system; mixtures; multiplexed fluorescence-based assays; nucleic acid assays; Energy exchange; Fluorescence; Microfluidics; Multiplexing; Probes; Proteins; Quantum dots;

fLanguage

English

Publisher

ieee

Conference_Titel

Information Photonics (IP), 2011 ICO International Conference on

Conference_Location

Ottawa, ON

Print_ISBN

978-1-61284-315-5

Type

conf

DOI

10.1109/ICO-IP.2011.5953741

Filename

5953741