Title :
Immobilized quantum dot bioprobes: Fiber optics and microfluidics for the development of nucleic acid assays
Author :
Tavares, A.J. ; Noor, O. ; Vannoy, C. ; Chen, L. ; Algar, W.R. ; Krull, U.J.
Author_Institution :
Dept. of Chem. & Phys. Sci., Univ. of Toronto Mississauga, Mississauga, ON, Canada
Abstract :
Multiplexed fluorescence-based assays are possible by immobilization of mixtures of different nucleic acid QD-bioprobes onto fused silica optical fibers, and onto glass surfaces of glass-PDMS microfluidic chips. Dynamic control of stringency by adjustment of the electrical potential to drive electroosmotic flow is advantageous. QD-bioprobes can be covalently anchored, or can be immobilized using a labile tethering system for removal and replacement.
Keywords :
II-VI semiconductors; bioMEMS; cadmium compounds; fluorescence; glass; macromolecules; microfluidics; mixtures; molecular biophysics; optical fibres; semiconductor quantum dots; silicon compounds; wide band gap semiconductors; zinc compounds; CdSe-ZnS; SiO2; covalently anchoring; electroosmotic flow; fiber optics; fused silica optical fibers; glass surfaces; glass-PDMS microfluidic chips; immobilized quantum dot bioprobes; labile tethering system; mixtures; multiplexed fluorescence-based assays; nucleic acid assays; Energy exchange; Fluorescence; Microfluidics; Multiplexing; Probes; Proteins; Quantum dots;
Conference_Titel :
Information Photonics (IP), 2011 ICO International Conference on
Conference_Location :
Ottawa, ON
Print_ISBN :
978-1-61284-315-5
DOI :
10.1109/ICO-IP.2011.5953741
