Monitoring light scattering characteristics of adherent cell cultures using a lab-on-a-chip

Author

Charwat, V. ; Muellner, P. ; Hainberger, R. ; Purtscher, M. ; Ertl, P. ; Tedde, S. ; Hayden, O.

Author_Institution

Health & Environ. Dept., AIT Austrian Inst. of Technol. GmbH, Vienna, Austria

fYear

2011

fDate

18-20 May 2011

Firstpage

1

Lastpage

2

Abstract

We have developed a μ-fluidic biochip capable of monitoring in real-time cellular phenotype dynamics by non-invasively assessing cell viability and reproduction over long periods of time. The μ-fluidic biochip contains integrated organic photodiodes that continuously perform light scattering measurements of adherent cell populations. In the presented work, we experimentally show that light scattering measurements are an effective label-free method for accurately assessing cell number variations. Furthermore, results obtained from 3D FDTD simulations indicate that side scatter characteristics of cells allow in principle predictions on cellular health status.

Keywords

bio-optics; cellular biophysics; finite difference time-domain analysis; lab-on-a-chip; light scattering; 3D FDTD simulation; adherent cell culture; cell viability; integrated organic photodiode; lab-on-a-chip; light scattering characteristic; light scattering measurement; microfluidic biochip capable; noninvasive assessment; real time cellular phenotype dynamics; Finite difference methods; Light scattering; Monitoring; Optical filters; Solid modeling; Three dimensional displays;

fLanguage

English

Publisher

ieee

Conference_Titel

Information Photonics (IP), 2011 ICO International Conference on

Conference_Location

Ottawa, ON

Print_ISBN

978-1-61284-315-5

Type

conf

DOI

10.1109/ICO-IP.2011.5953790

Filename

5953790