PDMS-Glass bonded Lab-on-a-chip device for Single Cell Analysis

Current paper describes the testing, alignment and bonding of a PDMS-glass bonded microfluidic device used for single cell analysis. The channels were successfully tested using carboxylate modified polystyrene beads for any leakage. Polystyrene beads of 2 mum have been successfully trapped within the reservoir of the device by well known dielectrophoresis technique and the same technique will be used to trap a typical yeast cell. Electrode configuration is the notable factor in which pre-sorter electrodes were introduced to bring the far-off particles to the trapping range. Currently work is being carried out on the viability of the yeast cell as PDMS by itself is hydrophobic. Channels are being tested by coating with a layer of PVA (polyvinyl alcohol) which can increase the time of hydrophilicity. At the same time the responses of the gold electrodes coated with PVA are studied as well.