Three-dimensional reconstruction of human median, ulnar and radial nerve

This study was undertaken to implement the three-dimensional (3D) reconstruction of the intraneural structure in the upper limb peripheral nerves. Three fresh cadaver peripheral nerves (one median, one ulnar and one radial nerve) were located with human hair, embedded in OCT, sectioned consecutively every 100 mum at a 20 mum thickness. Then all slices were stained with the acetylcholinesterase histochemical (AChe) method. 2D information of these slices were obtained through high resolution scanner. A special software (3D nerve visualization system) was used for 3D reconstruction of these nerves. Finally, 3D microstructure inside these nerves were reproduced in the computer. 3D nerve visualization system can show exactly the 3D microstructure inside these nerves and the rules of the regrouping and redistribution of the fasciculus. In addition, the reconstructed nerves could rotate at any angle along any axis, which might help us to observe the shape, position and relationships of fasciculus from different positions. It might do a great help to provide a anatomic basis for the approach in treating peripheral nerve injuries.