Nanobiophotonics: Using the nanophotonics toolbox to manipulate biological fluorophores

Author

Blum, Christian ; Zijlstra, Niels ; Cesa, Yanina ; Subramaniam, Vinod ; van den Broek, Johanna M. ; Mosk, Allard P. ; Vos, Willem L.

Author_Institution

Biomed. Technol. & Tech. Med., Univ. of Twente, Enschede, Netherlands

fYear

2011

fDate

8-10 June 2011

Firstpage

1

Lastpage

3

Abstract

We show that nanophotonic manipulation of biological fluorophores can alter the spectral characteristics of visible fluorescent proteins (VFP), and that the changed spectral properties quantitatively reflect the sub surface quality of photonic crystals. Nanophotonic manipulation of VFP fluorescence lifetimes gives access to the quantum efficiency of emitting states without biasing from dark states, a parameter inaccessible to conventional methods.

Keywords

dark states; fluorescence; macromolecules; molecular biophysics; molecule-photon collisions; nanobiotechnology; proteins; radiative lifetimes; VFP spectral characteristics alteration; biological fluorophore nanophotonic manipulation; dark states; nanobiophotonics; nanophotonics toolbox; photonic crystal subsurface quality; quantum efficiency; visible fluorescent proteins; Color; Dark states; Fluorescence; Nanophotonics; Photonic crystals; Proteins;

fLanguage

English

Publisher

ieee

Conference_Titel

BioPhotonics, 2011 International Workshop on

Conference_Location

Parma

Print_ISBN

978-1-4244-9836-9

Electronic_ISBN

978-1-4244-9835-2

Type

conf

DOI

10.1109/IWBP.2011.5954845

Filename

5954845