Influence of processing flaws on cytotoxicity and genotoxicity of Co-Cr and Ni-Cr based dental crowns and bridges

Co-Cr and Ni-Cr based dental metal alloys are often preferred in the restoration processes inside the mouth for repairing the esthetic and functional irregularities of teeth. In this study, the cytotoxic and genotoxic effects of the possible man-made errors in the production of the dental crowns and bridges from the mentioned casting alloys in-vitro were investigated applying the test protocols according to TS EN ISO 10993 standards. As the possible man-made errors in this production, the duration of oxy-acetylene welding flame applied during the melting stage of the process and polishing time during the polishing process are determined. Their 9 different combinations are the investigated parameters of the study. Results showed that the welding flame induces toxicity in both of the alloys. In the cytotoxicity tests, as the duration of oxy-acetylene welding flame exposure increase, the negative effects on viability and morphological changes of the cells are noticed. Also increasing polishing time in the production process induces cytotoxicity and genotoxicity of these dental alloys.