Tissue response during staining and illumination of voltage-sensitive dye in rabbit myocardium

Author

Nováková, M. ; Nogová, K. ; Bardonová, J. ; Provazník, I.

Author_Institution

Dept. of Physiol., Masaryk Univ. Brno, Brno

fYear

2007

fDate

Sept. 30 2007-Oct. 3 2007

Firstpage

665

Lastpage

668

Abstract

Voltage-sensitive dyes (VSDpsilas) undergo changes in their electronic structure, and consequently their fluorescence spectra, in response to changes in the surrounding electric field and are therefore used for recording of monophasic action potentials (MAPs) by optical method. S.-c. phototoxic effects of VSDs appear under intense illumination and include alterations in heart electrical signals. To examine putative side effects of VSD di-4-ANEPPS in rabbit hearts, we studied electrogram, mean coronary flow and formation of hydroxyl radicals in the control, staining and washout periods and during illumination. Possible ultrastructural changes were examined by electron microscopy. Rabbit myocardium is resistant to di-4-ANEPPS side effects during staining and also resistant to bleaching during intense illumination since only minor changes in ECG and no change in other parameters were observed.

Keywords

bioelectric potentials; biological tissues; biomedical measurement; dyes; electrocardiography; electron microscopy; fluorescence; haemodynamics; optical saturable absorption; VSD di-4-ANEPPS side effects; bleaching; di-4-amino-naphthyl-ethenyl-pyridinium; electrocardiography; electrogram; electron microscopy; fluorescence spectra; hydroxyl radicals formation; mean coronary flow; monophasic action potentials; rabbit hearts; rabbit myocardium; staining period; tissue response; voltage-sensitive dyes; washout period; Bleaching; Electron microscopy; Fluorescence; Heart; Lighting; Myocardium; Optical recording; Rabbits; Resistance; Voltage;

fLanguage

English

Publisher

ieee

Conference_Titel

Computers in Cardiology, 2007

Conference_Location

Durham, NC

ISSN

0276-6547

Print_ISBN

978-1-4244-2533-4

Electronic_ISBN

0276-6547

Type

conf

DOI

10.1109/CIC.2007.4745573

Filename

4745573