Title :
Temperature and hydration effect on the low-frequency dynamics of proteins
Author :
Kambara, Ohki ; Tominaga, Keisuke
Author_Institution :
Mol. Photoscience Res. Center, Kobe Univ., Kobe, Japan
Abstract :
Absorption coefficient and refractive index of hen egg white lysozyme have been obtained in the frequency region of 3-50 cm-1 to estimate reduced absorption cross section (RACS). Experimental conditions such as hydration and temperature were varied, and we found by using RACS analysis that high temperature and high hydration condition is needed for proteins to be set in the functional state.
Keywords :
molecular biophysics; proteins; solvation; terahertz wave spectra; RACS analysis; hen egg white lysozyme absorption coefficient; hen egg white lysozyme refractive index; hydration effects; low frequency protein dynamics; reduced absorption cross section; temperature effects; wave number 3 cm-1 to 50 cm-1; Amino acids; Electromagnetic wave absorption; Frequency estimation; Molecular biophysics; Powders; Proteins; Refractive index; Spectroscopy; Temperature measurement; Time domain analysis;
Conference_Titel :
Infrared, Millimeter, and Terahertz Waves, 2009. IRMMW-THz 2009. 34th International Conference on
Conference_Location :
Busan
Print_ISBN :
978-1-4244-5416-7
Electronic_ISBN :
978-1-4244-5417-4
DOI :
10.1109/ICIMW.2009.5325690
