Application of the holographic phase contrast method for 3D imaging of phase micro-objects

Author

Tishko, T.V. ; Titar, V.P. ; Tishko, D.N.

Author_Institution

V.N. Karazin Kharkov Nat. Univ., Ukraine

fYear

2004

fDate

6-9 Sept. 2004

Firstpage

254

Lastpage

256

Abstract

This study presents the results of application of the holographic phase contrast method for computer 3D imaging of native blood erythrocytes. In this method, phase contrast is obtained by holographic addition and subtraction of perturbed waves by the micro-objects and unperturbed waves of equal intensities in interference fringes. From the presented results, it can be concluded that the holographic phase contrast method can be effectively used for 3D imaging of phase micro-objects. The holographic interference microscope with computer processing of interferograms can be an effective device for the purposes of medical diagnostics and scientific investigations.

Keywords

biomedical optical imaging; blood; cellular biophysics; holographic interferometry; image reconstruction; optical microscopy; patient diagnosis; 3D imaging; computer processing; holographic addition; holographic interference microscope; holographic phase contrast method; holographic subtraction; interference fringes; interferograms; medical diagnostics; native blood erythrocytes; perturbed waves; phase microobjects; unperturbed waves; Blood; Chemicals; Holography; Interference; Microscopy; Morphology; Optical imaging; Optical interferometry; Organisms; Refractive index;

fLanguage

English

Publisher

ieee

Conference_Titel

Laser and Fiber-Optical Networks Modeling, 2004. Proceedings of LFNM 2004. 6th International Conference on

Print_ISBN

0-7803-8429-6

Type

conf

DOI

10.1109/LFNM.2004.1382481

Filename

1382481