Title :
Application of the holographic phase contrast method for 3D imaging of phase micro-objects
Author :
Tishko, T.V. ; Titar, V.P. ; Tishko, D.N.
Author_Institution :
V.N. Karazin Kharkov Nat. Univ., Ukraine
Abstract :
This study presents the results of application of the holographic phase contrast method for computer 3D imaging of native blood erythrocytes. In this method, phase contrast is obtained by holographic addition and subtraction of perturbed waves by the micro-objects and unperturbed waves of equal intensities in interference fringes. From the presented results, it can be concluded that the holographic phase contrast method can be effectively used for 3D imaging of phase micro-objects. The holographic interference microscope with computer processing of interferograms can be an effective device for the purposes of medical diagnostics and scientific investigations.
Keywords :
biomedical optical imaging; blood; cellular biophysics; holographic interferometry; image reconstruction; optical microscopy; patient diagnosis; 3D imaging; computer processing; holographic addition; holographic interference microscope; holographic phase contrast method; holographic subtraction; interference fringes; interferograms; medical diagnostics; native blood erythrocytes; perturbed waves; phase microobjects; unperturbed waves; Blood; Chemicals; Holography; Interference; Microscopy; Morphology; Optical imaging; Optical interferometry; Organisms; Refractive index;
Conference_Titel :
Laser and Fiber-Optical Networks Modeling, 2004. Proceedings of LFNM 2004. 6th International Conference on
Print_ISBN :
0-7803-8429-6
DOI :
10.1109/LFNM.2004.1382481
