Investigation of binding parameters of immunoglobulins to latex microspheres

Theoretical and experimental investigation of the process of adsorption of human immunoglobulins to latex microspheres was carried out. The theory is based on calculations of the grand partition function. Experiments were based on a Scatchard plot of binding isotherms of proteins to latex microspheres. The kinetics and temperature dependence of the binding process were investigated. We obtained the binding constant and number of binding sites for the binding of immunoglobulins to carboxylated polystyrene latex particles. We also investigated the influence of pH and ionic strength on this binding. For the binding of human IgG to carboxylated latex particles the binding constant decreases 5 times when pH is changing from 6.0 to 9.0. This approach may be useful for all types of latexes