Multiple DC field pulse mediated fusion of RBC with amnion

Author

Biswas, S. ; Guha, S.K. ; Anand, S.

Author_Institution

Center for Biomed. Eng., Indian Inst. of Technol., New Delhi, India

fYear

1995

fDate

15-18 Feb 1995

Abstract

Cells tightly contacted to tissue surface by mechanical pressure or AC dielectrophoresis can be fused by electropulsing on to the tissue membrane. We report a multiple pulse electrofusion process to fuse uncontacted human RBC directly on human term placetal amnion tissue in-vitro without any morphological damage to both the cell, and the tissue. 10 rectangular pulses of 10 μs pulse length and of electric field (EF) ranging from 0.2 to 0.3 kV/cm, can make RBC, suspended at the fetal side of the amnion, to fuse with the columnar or cuboidal epithelial cell´s membrane of the amnion

Keywords

bioelectric phenomena; biological techniques; biomembranes; cellular biophysics; electrophoresis; 10 mus; AC dielectrophoresis; RBC; amnion; biotechnology; cell biology; cells; columnar epithelial cell membrane; cuboidal epithelial cell membrane; drug delivery; electric field; electropulsing; fetal side; human term placetal amnion tissue; mechanical pressure; morphological damage; multiple DC field pulse mediated fusion; multiple pulse electrofusion process; pulse length; rectangular pulses; tissue membrane; tissue surface; uncontacted human RBC; Power supplies; Pulsed power supplies;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1995 and 14th Conference of the Biomedical Engineering Society of India. An International Meeting, Proceedings of the First Regional Conference., IEEE

Conference_Location

New Delhi

Print_ISBN

0-7803-2711-X

Type

conf

DOI

10.1109/RCEMBS.1995.533048

Filename

533048