Portable holgraphic imager for biological samples

A portable holographic imaging platform combining with an automatic micro-objects detection algorithm is demonstrated for biological samples. All components of the imaging platform are aligned to one another along the same optical axis, provides many advantages such as large field-of-view, simple optical setup, and no optical aberration. Main features in the software are feature detection and counting. The average overall computational time is of 52.37 seconds, specificity and sensitivity of our algorithms on a 2593×1944 pixels hologram are 0.978 and 0.923 respectively. Although the algorithm is not optimized, the technique here only focuses on the detection and counting problem.