Title :
Effect of magnetic fields on the enzymatic activity of plasmin
Author :
Iwasaka, Masakazu ; Ueno, Shoogo ; Tsuda, Hiroko
Author_Institution :
Dept. of Comput. Sci. & Commun. Eng., Kyushu Univ., Fukuoka, Japan
Abstract :
The authors investigated the effect of magnetic fields on the enzymatic activity of plasmin concerning the dissolution of thrombus. Plasmin activity in magnetic fields up to 8 T was assessed using a synthetic substrate for plasmin, D-Val-Leu-Lys-pNA. After 40 min or more incubation, absorbance of the mixture exposed to an 8 T magnetic field at 37°C was 7-11% lower than control sample. The results show that the enzymatic activity of plasmin was affected by a magnetic field at 8 T. This suggests that magnetic fields have an effect on the active center of the enzymatic reaction of plasmin with a substrate, especially on the hydrogen bonds of the charge-relay system
Keywords :
biological effects of fields; biomagnetism; biomolecular effects of radiation; magnetic field effects; proteins; 37 C; 40 min; 8 T; D-Val-Leu-Lys-pNA; active center; charge-relay system; enzymatic reaction; hydrogen bonds; plasmin enzymatic activity; synthetic substrate; thrombus dissolution; Amino acids; Biomedical engineering; Computer science; Degradation; Hospitals; Hydrogen; Laboratories; Magnetic field measurement; Magnetic fields; Superconducting magnets;
Conference_Titel :
Engineering in Medicine and Biology Society, 1994. Engineering Advances: New Opportunities for Biomedical Engineers. Proceedings of the 16th Annual International Conference of the IEEE
Conference_Location :
Baltimore, MD
Print_ISBN :
0-7803-2050-6
DOI :
10.1109/IEMBS.1994.415280
