New recording setup for ratiometric recording of action potentials by optical means

Monophasic action potential (MAP) can be recorded from the heart surface by optical method via fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which a new ratiometric recording setup is used for recording MAPs from isolated rabbit hearts perfused according to Langendorff. MAPs recorded by new ratiometric recording setup are compared with simple optical MAP measurement without ratiometry.