Title :
Living cell imaging by high resolution microscopy with two-photon excitation
Author :
Jiyao Chen ; Tao Wang
Author_Institution :
Dept. of Phys., Fudan Univ., Shanghai, China
Abstract :
In common fluorescence microscopy with one-photon excitations (OPE), the space resolution is about λ/2. Using femto-second (fs) laser, the two-photon excitation (TPE) can be achieved on the center part of the focused spot breaking the resolution limit producing the high resolution microscopy. In this work, the living cell imaging under OPE and TPE were comparatively measured. The autofluorescence interference of native fluorophores in living cells is serious under OPE, whereas the TPE of an 800 nm fs laser can produce a high space resolution images with good imaging quality by effectively eliminating the effect of autofluorescence.
Keywords :
biomedical optical imaging; cellular biophysics; fluorescence; image resolution; optical microscopy; photoexcitation; two-photon processes; autofluorescence interference; fluorescence microscopy; fluorophores; high resolution microscopy; high space resolution images; imaging quality; living cell imaging; one-photon excitations; two-photon excitation; Fluorescence; Image resolution; Measurement by laser beam; Microscopy; Probes; Quantum dot lasers; Femto-second laser; high resolution microscopy; imaging measurement; quantum dots; two-photon excitation;
Conference_Titel :
Precision Electromagnetic Measurements (CPEM 2014), 2014 Conference on
Conference_Location :
Rio de Janeiro
Print_ISBN :
978-1-4799-5205-2
DOI :
10.1109/CPEM.2014.6898388
