DocumentCode :
2368012
Title :
Force characterization of live cells in automated transportation with robot-tweezers manipulation system
Author :
Wu, Yanhua ; Chen, Haoyao ; Sun, Dong ; Huang, Wenhao
Author_Institution :
Mech. & Autom. Group, City Univ. of Hong Kong, Suzhou, China
fYear :
2010
fDate :
4-7 Aug. 2010
Firstpage :
1913
Lastpage :
1918
Abstract :
Optical trap provides a new and unique tool for manipulation in micro/nano scale and have wide applications in biological fields. Increasing demands for both accuracy and efficiency in cell manipulation highlight the need for automation process that integrates robotics and optical tweezers technologies. A clear understanding of the forces exerted on cells, i.e., trapping and viscous drag forces, is essential to enable automated manipulation using optical tweezers. In this paper, we propose a method to characterize the forces exerted on the live cell moved by optical trap, where the cell is bound to a micro polystyrene bead. Experiment of the cell falling to the focus of the optical trap is performed to show that the proposed force calibration method exhibit better accuracy than the traditional ray-optic numerical method based on the transparent-bead model. Further, automated transportation of the yeast cell is performed to demonstrate the effectiveness in applying the proposed force characterization approach to automated cell manipulation.
Keywords :
biocontrol; cellular transport; force measurement; micromanipulators; radiation pressure; automated transportation; force calibration method; force characterization; live cell; micro polystyrene bead; optical trap; robot tweezer manipulation system; yeast cell; Biomedical optical imaging; Calibration; Charge carrier processes; Drag; Force; Power lasers;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Mechatronics and Automation (ICMA), 2010 International Conference on
Conference_Location :
Xi´an
ISSN :
2152-7431
Print_ISBN :
978-1-4244-5140-1
Electronic_ISBN :
2152-7431
Type :
conf
DOI :
10.1109/ICMA.2010.5588938
Filename :
5588938
Link To Document :
بازگشت