Direct electrochemistry of horseradish peroxidase in layer-by-layer nanotubes synthesized on template

Horseradish peroxidase (HRP) and phthalic diglycol diacrylate (PDDA) was successfully immobilized on anodic aluminum oxide (AAO) template modified glassy carbon (GC) electrode using electric field directed layer-by-layer assembly (EFDLA) synthesis. The materials were characterized by scanning electric microscopy (SEM), transmission electric microscopy (TEM), UV-vis spectroscopy (UV-vis) and electrochemical impedance spectroscopy (EIS). A pair of redox peaks resulted from the redox reaction of IMP were observed with the formal potential of 0.012 V (v.s. SCE) in 0.2 mol/L pH=7.0 phosphate buffer solution (PBS). The number of transference electron was 1.05 and the direct electron transfer (eT) constant (k_s) was 0.59 s^-1.