Title :
Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment
Author :
Lesuffleur, Antoine ; Lim, Kwan Seop ; Lindquist, Nathan C. ; Im, Hyungsoon ; Warrington, Arthur E. ; Rodriguez, Moses ; Oh, Sang-Hyun
Author_Institution :
Dept. of Electr. & Comput. Eng., Univ. of Minnesota, Minneapolis, MN, USA
Abstract :
We integrate periodic nanohole arrays in a thin gold film with lipid membranes in a microfluidic channel. Surface plasmon-enhanced light transmission through the periodic nanohole arrays enables real-time label-free sensing of molecular binding on the lipid membrane surface. This membrane biosensor can potentially act as a natural platform for studying binding kinetics of proteins with their binding partners anchored in the lipid membrane. We also present the concept of using nanopore arrays for kinetic assays of transmembrane proteins in a free-standing lipid membrane.
Keywords :
bio-optics; biomembrane transport; biosensors; gold; lipid bilayers; microfluidics; microsensors; molecular biophysics; nanobiotechnology; nanosensors; optical sensors; plasmonics; proteins; surface plasmons; thin film sensors; Au; free-standing lipid membrane environment; integrated periodic nanohole arrays; label-free kinetic biosensing; lipid membrane biosensor; microfluidic channel; molecular binding; plasmonic nanohole arrays; proteins binding kinetics; real-time label-free sensing; surface plasmon-enhanced light transmission; thin gold film; transmembrane protein kinetic assays; Biosensing Techniques; Kinetics; Membrane Lipids; Microscopy, Electron, Scanning; Surface Plasmon Resonance;
Conference_Titel :
Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE
Conference_Location :
Minneapolis, MN
Print_ISBN :
978-1-4244-3296-7
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2009.5332429
