An automatic Method to identify and extract information of DNA bands in Gel Electrophoresis images

Author

Caridade, C.M.R. ; Marcal, Andre R. S. ; Mendonca, Teresa ; Pessoa, A.M. ; Pereira, S.

Author_Institution

Inst. Super. de Eng. de Coimbra, Coimbra, Portugal

fYear

2009

fDate

3-6 Sept. 2009

Firstpage

1024

Lastpage

1027

Abstract

This paper presents a system for the automatic processing of digital images obtained from gel electrophoresis. The system identifies automatically the number and the location of lanes in the digital image, as well as the location of bands on each lane, without any intervention from the user. A reference lane with a know substance is used to compute the molecular weight of the observed (unknown) bands. The system performance was tested using 12 images, obtained from 4 gels with 3 different exposures. A total of 5443 bands were tested in 12 images, 672 reference / observed lane pairs. The average error in the estimation of molecular weight of 9.2%.

Keywords

DNA; electrophoresis; feature extraction; gels; genomics; image processing; molecular biophysics; DNA; digital images; gel electrophoresis; Algorithms; Artificial Intelligence; DNA; Electrophoresis, Gel, Two-Dimensional; Image Enhancement; Image Interpretation, Computer-Assisted; Microscopy, Fluorescence; Pattern Recognition, Automated; Reproducibility of Results; Sensitivity and Specificity;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE

Conference_Location

Minneapolis, MN

ISSN

1557-170X

Print_ISBN

978-1-4244-3296-7

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/IEMBS.2009.5332462

Filename

5332462