Title :
Widefield fluorescence sectioning with HiLo microscopy
Author :
Mertz, Jerome ; Lim, Daryl ; Chu, Kengyeh K. ; Bozinovic, Nenad ; Ford, Timothy
Author_Institution :
Dept. of Biomed. Eng., Boston Univ., Boston, MA, USA
Abstract :
HiLo microscopy is a widefield fluorescence imaging technique that provides depth discrimination by combining two images, one with non-uniform illumination and one with uniform illumination. We discuss the theory of this technique and a variety of practical implementations in brain-tissue imaging and fluorescence endomicroscopy.
Keywords :
biomedical optical imaging; brain; fluorescence; optical microscopy; HiLo microscopy; brain-tissue imaging; depth discrimination; fluorescence endomicroscopy; nonuniform illumination; uniform illumination; widefield fluorescence sectioning; Animals; Equipment Design; Humans; Image Processing, Computer-Assisted; Microscopy, Confocal; Microscopy, Fluorescence; Optics and Photonics; Reproducibility of Results;
Conference_Titel :
Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE
Conference_Location :
Minneapolis, MN
Print_ISBN :
978-1-4244-3296-7
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2009.5333166
