Title :
Application of multiphoton excitation-evoked chromophore-assisted laser inactivation (MP-CALI) for analysis of chromosomal passenger protein function
Author :
Harada, Yoshinori ; Dai, Ping ; Takamatsu, Tetsuro
Author_Institution :
Dept. of Pathology & Cell Regul., Kyoto Prefectural Univ. of Med., Kyoto
Abstract :
Recently, we have developed a technology which can specifically inactivate intracellular protein molecules with high spatial and time resolution by using femtosecond pulse laser and green fluorescent protein, i.e., multiphoton excitation-evoked chromophore-assisted laser inactivation (MP-CALI). To analyze precise functions of chromosomal passenger proteins, we conduct experiments by applying the method of MP-CALI.
Keywords :
biological effects of laser radiation; cellular biophysics; fluorescence; genetics; molecular biophysics; multiphoton processes; proteins; MP-CALI; chromosomal passenger protein function; femtosecond pulse laser; green fluorescent protein; high spatial resolution; intracellular protein molecules; multiphoton excitation-evoked chromophore-assisted laser inactivation; time resolution; Biological cells; Fluorescence; Laser excitation; Microscopy; Network address translation; Optical wavelength conversion; Power lasers; Protein engineering; Spatial resolution; Ultrafast optics;
Conference_Titel :
Micro-NanoMechatronics and Human Science, 2008. MHS 2008. International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4244-2918-9
Electronic_ISBN :
978-1-4244-2919-6
DOI :
10.1109/MHS.2008.4752476
