DocumentCode
2398843
Title
All optical histology: serial ablation and multiphoton imaging of neuronal tissue with femtosecond laser pulses
Author
Tsai, Philbert S. ; Friedman, Beth ; Uerri, Agustin I Ifarra ; Thompson, Beverly D. ; Lev-Ram, Varda ; Schaffer, Chris B. ; Xiong, Qing ; Tsie, Roger Y. ; Squier, Jeffrey A. ; Kleinfeld, David
Author_Institution
Dept. of Phys., California Univ., USA
Volume
2
fYear
2003
fDate
27-28 Oct. 2003
Firstpage
485
Abstract
We use femtosecond laser pulses to serially image and ablate neuronal tissue. This allows histological imaging throughout the entire depth of the brain; a procedure that is unattainable by multiphoton imaging alone.
Keywords
biological tissues; biomedical optical imaging; brain; high-speed optical techniques; laser ablation; laser applications in medicine; neurophysiology; all optical histology; brain; femtosecond laser pulses; histological imaging; laser ablation; multiphoton imaging; neuronal tissue; Fluorescence; Image reconstruction; Laser ablation; Mice; Optical buffering; Optical imaging; Optical pulses; Optical scattering; Proteins; Ultrafast optics;
fLanguage
English
Publisher
ieee
Conference_Titel
Lasers and Electro-Optics Society, 2003. LEOS 2003. The 16th Annual Meeting of the IEEE
ISSN
1092-8081
Print_ISBN
0-7803-7888-1
Type
conf
DOI
10.1109/LEOS.2003.1252885
Filename
1252885
Link To Document