Title :
All optical histology: serial ablation and multiphoton imaging of neuronal tissue with femtosecond laser pulses
Author :
Tsai, Philbert S. ; Friedman, Beth ; Uerri, Agustin I Ifarra ; Thompson, Beverly D. ; Lev-Ram, Varda ; Schaffer, Chris B. ; Xiong, Qing ; Tsie, Roger Y. ; Squier, Jeffrey A. ; Kleinfeld, David
Author_Institution :
Dept. of Phys., California Univ., USA
Abstract :
We use femtosecond laser pulses to serially image and ablate neuronal tissue. This allows histological imaging throughout the entire depth of the brain; a procedure that is unattainable by multiphoton imaging alone.
Keywords :
biological tissues; biomedical optical imaging; brain; high-speed optical techniques; laser ablation; laser applications in medicine; neurophysiology; all optical histology; brain; femtosecond laser pulses; histological imaging; laser ablation; multiphoton imaging; neuronal tissue; Fluorescence; Image reconstruction; Laser ablation; Mice; Optical buffering; Optical imaging; Optical pulses; Optical scattering; Proteins; Ultrafast optics;
Conference_Titel :
Lasers and Electro-Optics Society, 2003. LEOS 2003. The 16th Annual Meeting of the IEEE
Print_ISBN :
0-7803-7888-1
DOI :
10.1109/LEOS.2003.1252885
