Label-free analysis of DNA methylation using optofluidic ring resonators

Author

Suter, Jonathan D. ; Howard, Daniel J. ; Shi, Huidong ; Caldwell, Charles W. ; Fan, Xudong

Author_Institution

Dept. of Biol. Eng., Univ. of Missouri, Columbia, MO, USA

fYear

2009

fDate

3-6 Sept. 2009

Firstpage

2760

Lastpage

2762

Abstract

We demonstrate the utility of the opto-fluidic ring resonator (OFRR) sensor for analyzing methylated oligonucleotides. Cytosine methylation, a regular epigenetic function in cellular growth and metabolism, may have ties to abnormal suppression of key genes involved with cellular proliferation. Such behavior is suspected to be strongly related to the occurrence of several types of cancers. The OFRR is demonstrated as a tool both for detecting DNA hybridization and methylated cytosines residues.

Keywords

DNA; bioMEMS; biochemistry; biosensors; microfluidics; micromechanical resonators; DNA methylation; cancer; cellular growth; cellular proliferation; cytosine methylation; gene suppression; label free analysis; metabolism; oligonucleotides; optofluidic ring resonators sensor; Animals; Cell Proliferation; Cytosine; DNA Methylation; Epigenesis, Genetic; Equipment Design; Humans; Methylation; Mice; Neoplasms; Nucleic Acid Hybridization; Oligonucleotides; Optics and Photonics;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE

Conference_Location

Minneapolis, MN

ISSN

1557-170X

Print_ISBN

978-1-4244-3296-7

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/IEMBS.2009.5333866

Filename

5333866