Development of Computer Assisted Sperm Analysis (CASA) plugin for analyzing sperm in microfluidic environments using Image-J

Author

Elsayed, Mohamed ; El-Sherry, Taymour ; Abdelgawad, Mohamed

Author_Institution

Assiut Univ., Assiut, Egypt

fYear

2014

fDate

11-13 Dec. 2014

Firstpage

149

Lastpage

152

Abstract

We modified a previously reported Computer Assisted Sperm Analysis (CASA) plugin for Image-J to enable analyzing sperm cells in microfluidic environments. An image processing pipeline was built for object detection and particle tracking was improved. New parameters were introduced to allow studying new aspects of sperm motion behavior such as rheotaxis and wall tracking. A preliminary study using this tool agreed well with previously reported studies on rheotaxis and wall tracking behavior of sperm.

Keywords

bioMEMS; biomedical optical imaging; cellular biophysics; medical image processing; microfluidics; object detection; CASA; Image-J; computer assisted sperm analysis; image processing pipeline; microfluidic environments; object detection; particle tracking; rheotaxis; sperm cells; sperm motion behavior; wall tracking behavior; Lithography; Microscopy; Optical imaging; Optical recording; Ultraviolet sources; CASA; Image-J; image processing sperm analysis; microfluidics; open source; particle detection; particle tracking;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering Conference (CIBEC), 2014 Cairo International

Conference_Location

Giza

ISSN

2156-6097

Print_ISBN

978-1-4799-4413-2

Type

conf

DOI

10.1109/CIBEC.2014.7020941

Filename

7020941