Title :
Quantum dots in molecular detection of disease
Author :
Bailey, V.J. ; Puleo, C.M. ; Ho, Y.P. ; Yeh, H.C. ; Wang, T.H.
Author_Institution :
Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD, USA
Abstract :
The unique photophysical properties of semiconductor quantum dots (QDs) have made them ideal for use as spectral labels and luminescent probes. In this review, applications are presented in which QDs function as active participants in nanoscale biosensor assemblies, where replacing traditional molecular fluorophores results in improved assay performance. Specific focus is on disease detection with applications including multiplexed target detection, mutation detection by coincidence analysis and QD-based FRET reporters for miRNA detection and DNA methylation analysis.
Keywords :
DNA; bioluminescence; biosensors; cellular biophysics; diseases; molecular biophysics; nanosensors; semiconductor quantum dots; DNA methylation analysis; disease detection; luminescent probes; miRNA detection; molecular detection; molecular fluorophores; multiplexed target detection; mutation detection; nanoscale biosensor assemblies; photophysical properties; semiconductor quantum dots; spectral labels; Alleles; Anthrax; Biosensing Techniques; DNA Methylation; DNA Mutational Analysis; Dose-Response Relationship, Drug; Equipment Design; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Humans; MicroRNAs; Models, Genetic; Nanotechnology; Oligonucleotides; Quantum Dots;
Conference_Titel :
Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE
Conference_Location :
Minneapolis, MN
Print_ISBN :
978-1-4244-3296-7
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2009.5334553
