Quantum dots in molecular detection of disease

Author

Bailey, V.J. ; Puleo, C.M. ; Ho, Y.P. ; Yeh, H.C. ; Wang, T.H.

Author_Institution

Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD, USA

fYear

2009

fDate

3-6 Sept. 2009

Firstpage

4089

Lastpage

4092

Abstract

The unique photophysical properties of semiconductor quantum dots (QDs) have made them ideal for use as spectral labels and luminescent probes. In this review, applications are presented in which QDs function as active participants in nanoscale biosensor assemblies, where replacing traditional molecular fluorophores results in improved assay performance. Specific focus is on disease detection with applications including multiplexed target detection, mutation detection by coincidence analysis and QD-based FRET reporters for miRNA detection and DNA methylation analysis.

Keywords

DNA; bioluminescence; biosensors; cellular biophysics; diseases; molecular biophysics; nanosensors; semiconductor quantum dots; DNA methylation analysis; disease detection; luminescent probes; miRNA detection; molecular detection; molecular fluorophores; multiplexed target detection; mutation detection; nanoscale biosensor assemblies; photophysical properties; semiconductor quantum dots; spectral labels; Alleles; Anthrax; Biosensing Techniques; DNA Methylation; DNA Mutational Analysis; Dose-Response Relationship, Drug; Equipment Design; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Humans; MicroRNAs; Models, Genetic; Nanotechnology; Oligonucleotides; Quantum Dots;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE

Conference_Location

Minneapolis, MN

ISSN

1557-170X

Print_ISBN

978-1-4244-3296-7

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/IEMBS.2009.5334553

Filename

5334553