Degradable polymers for gene delivery

Author

Sunshine, Joel ; Bhise, Nupura ; Green, Jordan J.

Author_Institution

Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD, USA

fYear

2009

fDate

3-6 Sept. 2009

Firstpage

2412

Lastpage

2415

Abstract

Degradable polymers were synthesized that self-assemble with DNA to form particles that are effective for gene delivery. Small changes to polymer synthesis conditions, particle formulation conditions, and polymer structure led to significant changes to efficacy in a cell-type dependent manner. Polymers presented here are more effective than Lipofectamine 2000 or polyethylenimine for gene delivery to cancerous fibroblasts or human primary fibroblasts. These materials may be useful for cancer therapeutics and regenerative medicine.

Keywords

DNA; biodegradable materials; cancer; cellular biophysics; gene therapy; materials preparation; polymer structure; tumours; DNA self-assembly; Lipofectamine 2000; cancer therapeutics; cancerous fibroblasts; cell-type dependent manner; degradable polymer synthesis condition; gene delivery; human primary fibroblasts; particle formulation condition; polyethylenimine; polymer structure; regenerative medicine; Animals; Biodegradation, Environmental; COS Cells; Cell Line, Tumor; Cercopithecus aethiops; Drug Carriers; Fibroblasts; Gene Therapy; Gene Transfer Techniques; Humans; Lipids; Neoplasms; Polyethyleneimine; Polymers; Regenerative Medicine;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE

Conference_Location

Minneapolis, MN

ISSN

1557-170X

Print_ISBN

978-1-4244-3296-7

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/IEMBS.2009.5334767

Filename

5334767