Non-Crosshybridizing Oligonucleotide Building Blocks for Accurate, Scalable Nanofabrication

The urgent need in the field of nanotechnology is functional, reliable, and scalable techniques for more complicated and controlled nanostructures in order to realize nanoscale materials and devices that have practical use and more capability than existing technologies. This paper addresses this need by presenting an in vitro protocol to select large non-crosshybridizing (NCH) libraries of oligonucleotides that provide a large address space of DNA sequences with which to increase both complexity and control in DNA-directed self-assembly processes. The selection protocol was experimentally verified and confirmed. The resultant large NCH libraries would allow self-assembling complex nanostructures with more precise control, leading to reliable, scaled-up, cost-effective manufacturing of nanoscale devices and systems.