Nonlinear Transmembrane Current Response of Micro Elelectroporation for Human Cancer Cells

This paper presents a method to quantify the nonlinear electric current responses of electroporation (EP) on microchips for human cancer cells. Micro EP chips with three dimensional gold electrodes were fabricated with a modified electroplating process. Electrochemical impedance spectroscopy (EIS) was carried out with an electrochemistry analyzer on micro EP chips and a nonlinear equivalent circuit model was proposed to describe the dynamic response of the whole system. Using such a method, micro EP current was isolated from undesired leakage current to study the corresponding nano electropore dynamics under different input voltages. In addition, the EP efficiency results from electric measurements were confirmed by studies using fluorescence microscopy.