Title :
Investigations of bio markers for human lymphoblastoid cells using Atomic Force Microscopy
Author :
Yang, Ruiguo ; Xi, Ning ; Fung, Carmen Kar Man ; Lai, King Wai Chiu ; Seiffert-Sinha, Kristina ; Sinha, Animesh A. ; Zhang, Weijing
Author_Institution :
Dept. of Electr. & Comput. Eng., Michigan State Univ., East Lansing, MI, USA
Abstract :
We report the use of Atomic Force Microcopy (AFM) in combination with Fluorescence Microscopy (FM) for the investigation of non-Hodgkin´s type B cells lymphoma after antibody treatment. Rituximab is used in the treatment this cancer type. It is a chimeric monoclonal antibody directed against the protein Cluster of Differentiation 20 (CD20), which is expressed on mature B cells, including the B cell lymphoma surface. We applied anti-CD20 antibody to the B lymphoma cells that was labeled with fluorophore. Simultaneous imaging was then performed by both FM and AFM. Immunofluorescence imaging confirms the binding of Rituximab to the CD20 protein; while the AFM imaging revealed changes in lymphoma cells in terms of mean cell height and cell surface roughness. In addition, our AFM based nanomanipulation system can assist in the analysis of mechanical property of the cell by recording the force-displacement curves at the cell surface. AFM imaging and measurements may provide biomarkers of cell behavior and may facilitate the treatment of lymphoma clinically as a pretest to determine the effectiveness of the drug Rituximab on lymphoma cells from individual patients.
Keywords :
atomic force microscopy; biochemistry; biomechanics; biomedical optical imaging; bonds (chemical); cancer; cellular biophysics; molecular biophysics; optical microscopy; patient treatment; proteins; surface roughness; AFM; CD20 protein; Rituximab binding; antibody treatment; atomic force microscopy; biomarkers; cancer type treatment; cell height; cell surface roughness; fluorescence microscopy; fluorophore; force-displacement curves; human lymphoblastoid cells; immunofluorescence imaging; mechanical property; monoclonal antibody; nanomanipulation system; non-Hodgkin type B cell lymphoma; protein cluster-of-differentiation; AFM live cell imaging; Atomic Force Microscopy; Fluorescence Microscopy; Rituximab; cell immobilization; human lymphoblastoid cells;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems (NEMS), 2010 5th IEEE International Conference on
Conference_Location :
Xiamen
Print_ISBN :
978-1-4244-6543-9
DOI :
10.1109/NEMS.2010.5592162
