Title :
Photothermal detection and tracking of individual non-fluorescent nano-objects in live cells
Author :
Cognet, L. ; Octeau, V. ; Lasne, D. ; Berciaud, S. ; Lounis, B.
Author_Institution :
Centre de Phys. Moleculaire Opt. et Hertzienne, CNRS, Talence
Abstract :
To overcome the photobleaching problem inherent to fluorescence techniques we recently developed a new optical detection method for individual non-fluorescent nano-objects. It allows detecting the movement of individual membrane proteins labeled with 5 nm gold nanoparticles in living cells for arbitrary long times.
Keywords :
nanobiotechnology; nanoparticles; photothermal effects; proteins; membrane proteins; nanoparticles; nonfluorescent nanoobjects; optical detection; photobleaching; photothermal detection; photothermal tracking; Absorption; Biomembranes; Fluorescence; Gold; Nanobioscience; Nanoparticles; Optical refraction; Optical scattering; Particle scattering; Rayleigh scattering;
Conference_Titel :
IEEE/LEOS Summer Topical Meetings, 2008 Digest of the
Conference_Location :
Acapulco
Print_ISBN :
978-1-4244-1925-8
Electronic_ISBN :
1099-4742
DOI :
10.1109/LEOSST.2008.4590491
