Enhanced optical micromanipulation and transfection of cells using femtosecond lasers

Author

Brown, C.T.A. ; Tsampoula, X. ; Stevenson, D. ; McDougall, C. ; Gunn-Moore, F.J. ; Dholakia, K.

Author_Institution

Sch. of Phys. & Astron., Univ. of St. Andrews, St. Andrews

fYear

2008

fDate

21-23 July 2008

Abstract

This work investigates the use of continuous-wave and femtosecond-pulsed modes of a laser to obtain different functionalities when interacting with living cells using a single, simple, optical setup. This study demonstrates that careful control of the laser source in both spatial and/or temporal aspects of the output beam can offer major advantages in enhanced functionality and ease of use of fs-optical transfection systems. The results of this study are applicable in systems where fs-lasers are being utilized in applications that rely on nonlinear effects.

Keywords

biological techniques; cellular biophysics; high-speed optical techniques; laser beam applications; laser modes; nonlinear optics; continuous-wave mode; femtosecond lasers; femtosecond-pulsed mode; laser modes; laser source control; living cells; nonlinear effects; optical micromanipulation; optical transfection systems; Biomembranes; Cells (biology); Laser applications; Laser beams; Laser modes; Nonlinear optics; Ultrafast optics;

fLanguage

English

Publisher

ieee

Conference_Titel

IEEE/LEOS Summer Topical Meetings, 2008 Digest of the

Conference_Location

Acapulco

ISSN

1099-4742

Print_ISBN

978-1-4244-1925-8

Type

conf

DOI

10.1109/LEOSST.2008.4590496

Filename

4590496