DocumentCode
2433280
Title
Image-based cell sorting using optofluidics
Author
Kovac, J.R. ; Voldman, J.
Author_Institution
Massachusetts Inst. of Technol., Cambridge, MA
fYear
2008
fDate
21-23 July 2008
Abstract
We present an architecture ideally suited for imaging and sorting large populations of single cells. Our approach combines wide-field, passive, hydrodynamic trapping of cells with active retrieval of specific single cells using radiation pressure and microfluidics. The architecture scales easily and offers single-cell resolution both with respect to imaging and manipulation, enabling sorts predicated on microscopy.
Keywords
biological techniques; cellular biophysics; fluorescence; integrated optics; micro-optomechanical devices; microfluidics; optical microscopy; radiation pressure; cell manipulation; fluorescence-activated cell sorting; hydrodynamic cells trapping; image-based cell sorting; microfluidic flow chamber; microfluidics; microscopy; optofluidics; radiation pressure; single-cell resolution; Arrays; Computer architecture; Fluorescence; Imaging; Microprocessors; Performance evaluation; Sorting;
fLanguage
English
Publisher
ieee
Conference_Titel
IEEE/LEOS Summer Topical Meetings, 2008 Digest of the
Conference_Location
Acapulco
ISSN
1099-4742
Print_ISBN
978-1-4244-1925-8
Type
conf
DOI
10.1109/LEOSST.2008.4590555
Filename
4590555
Link To Document