Title :
Cloning and expressing an OsDCL3b gene from Oryza sativa indica
Author :
Peng, Xiaojue ; Yu, Chao ; Li, Shaobo ; Zhu, Youlin ; Chen, Zuyu
Author_Institution :
Sch. of Life Sci. & Food Eng., Nanchang Univ., Nanchang, China
Abstract :
A full length dicer-like gene was cloned from 9311 (Oryza sativa indica) by RACE (GenBank accession No: DQ208406). It should be an OsDCL3b through homogeneous alignment and locate on chromosome 10. This gene might have alternative splicing transcripts. Real-time PCR results indicated that it expressed at different levels in root, stem, leaf and flower, respectively. The highest expression level of this gene was detected in flower, while the lowest expression level was detected in root.
Keywords :
DNA; botany; genetics; molecular biophysics; Oryza sativa indica; OsDCL3b gene cloning; OsDCL3b gene expression; RACE; Real-time PCR; alternative splicing transcripts; chromosome; flower; full length dicer-like gene; homogeneous alignment; leaf; root; stem; Animals; Cloning; Materials; Proteins; RNA; Real time systems; Splicing; Oryza sativa indica; OsDCL3; Real-time PCR; alternative splicing;
Conference_Titel :
Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on
Conference_Location :
Nanjing
Print_ISBN :
978-1-4244-9172-8
DOI :
10.1109/RSETE.2011.5964037
