Research on salt-tolerant gene HOG1 in Torulopsis versatilis

Author

Cao, Xiaohong ; Wang, Yuanyuan ; Hou, Lihua

Author_Institution

Key Lab. of Food Nutrition & Safety, Tianjin Univ. of Sci. & Technol., Tianjin, China

fYear

2011

fDate

24-26 June 2011

Firstpage

8454

Lastpage

8456

Abstract

To better understand the osmo-adaption mechanism in the Torulopsis versatilis, we investigated the salt-tolerant gene HOG1 from the wild-type T. versatilis. Genome of T yeast was used for PCR reaction to amplify the HOG1 gene. The PCR productions were sent into sequencing vector pUC19 to achieve the plasmid pUC19-HOG1. The engineered strain W303-YEp195-T and the control strain W303-YEp195 were constructed, respectively. It was found that overexpression of THOG1 could obviously increase the tolerance of salt, compared to the control. Identification and overexpression of THOG1 play an important role for the osmo-adaption mechanism of the T. versatilis used in soy-sauce fermentation.

Keywords

fermentation; food products; food technology; genetic engineering; microorganisms; HOG1 gene; PCR productions; PCR reaction; T yeast; THOG1; Torulopsis versatilis; W303-YEp195-T; control strain; engineered strain; osmo-adaption mechanism; plasmid pUC19-HOG1; salt tolerance; salt-tolerant gene; sequencing vector; soy-sauce fermentation; wild-type T. versatilis; Cloning; DNA; Proteins; RNA; Sequences; Strain; Stress; HOG1; Salt tolerance; Torulopsis versatilis;

fLanguage

English

Publisher

ieee

Conference_Titel

Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on

Conference_Location

Nanjing

Print_ISBN

978-1-4244-9172-8

Type

conf

DOI

10.1109/RSETE.2011.5964131

Filename

5964131