Fuzzy enhancement for nonuniform illumination of microscopic Sprague Dawley rat sperm image

This paper presents a fuzzy grayscale enhancement technique for nonuniform illumination of microscopic Sprague Dawley rat sperm image. The microscopic images extracted from the sperm motility analysis video are low in contrast and having nonuniform lighting. Most of the developed techniques enhanced the microscopic image without considering nonuniform brightness in the image. Thus, overenhanced or underenhanced phenomena in the processed image are inevitable. The fuzzy grayscale image enhancement technique is proposed to overcome the aforementioned problems. The enhancement process of sperm images is conducted according to predetermined overexposed and underexposed regions. The proposed method has attained optimum fuzziness measures and the quality of the sperm image is improved. In addition, the proposed method required minimum processing time as compared to the other methods.