Efficiency of polymerase chain reaction processes: A stochastic model

Author

Hassibi, Arjang ; Sharif, Masoud

Author_Institution

California Inst. of Technol., Pasadena, CA

fYear

2006

fDate

28-30 May 2006

Firstpage

35

Lastpage

36

Abstract

A stochastic model for the efficiency of polymerase chain reaction (PCR) processes is presented. The model is based on the assumption that the i´th nucleotide incorporation on the DNA template takes tau_i seconds, where tau_i has an exponential distribution with mean 1/lambda_i. In this paper, given lambda_i that can be obtained from the primer-template sequence, temperature profile, enzyme rate, and other assay conditions, we calculate the efficiency of a multi-step PCR process using the distribution of the summation of non-identical exponential distributions.

Keywords

DNA; biochemistry; biothermics; enzymes; molecular biophysics; physiological models; DNA; enzyme rate; nucleotide; polymerase chain reaction; primer-template sequence; stochastic model; temperature profile; Analytical models; Biochemistry; DNA; Exponential distribution; Impedance; Polymers; Probability distribution; Sequences; Stochastic processes; Temperature distribution;

fLanguage

English

Publisher

ieee

Conference_Titel

Genomic Signal Processing and Statistics, 2006. GENSIPS '06. IEEE International Workshop on

Conference_Location

College Station, TX

Print_ISBN

1-4244-0384-7

Electronic_ISBN

1-4244-0385-5

Type

conf

DOI

10.1109/GENSIPS.2006.353143

Filename

4161764