Title :
On joint maximum-likelihood estimation of PCR efficiency and initial amount of target
Author :
Vikalo, H. ; Hassibi, B. ; Hassibi, A.
Author_Institution :
California Inst. of Technol., Pasadena, CA
Abstract :
We consider the problem of estimating unknown parameters of the real-time polymerase chain reaction (RT- PCR) from noisy observations. The joint ML estimator of the RT-PCR efficiency and the initial number of DNA target molecules is derived. The mean-square error performance of the estimator is studied via simulations. The simulation results indicate that the proposed estimator significantly outperforms a competing technique.
Keywords :
DNA; biochemistry; biology computing; maximum likelihood estimation; mean square error methods; molecular biophysics; PCR; initial DNA target molecule; joint maximum-likelihood estimation; mean-square error performance; real-time polymerase chain reaction; Additive noise; Annealing; Biochemistry; DNA; In vitro; Maximum likelihood estimation; Noise measurement; Parameter estimation; Polymers; Temperature;
Conference_Titel :
Genomic Signal Processing and Statistics, 2006. GENSIPS '06. IEEE International Workshop on
Conference_Location :
College Station, TX
Print_ISBN :
1-4244-0384-7
Electronic_ISBN :
1-4244-0385-5
DOI :
10.1109/GENSIPS.2006.353149