Title :
Interdigitated electrodes based on impedance biosensor for sensing peptide LL-37
Author :
Lee, Jia-Yi ; Wang, Chung-Yih ; Huang, Chi-Fang ; Cheng, An-Ting
Author_Institution :
Institute of Electrical Engineering, Tatung University, 40, Chung-Shan N. Rd., Sec. 3, Taipei 104, Taiwan
fDate :
Aug. 30 2011-Sept. 3 2011
Abstract :
An antimicrobial peptide, LL-37, is found in an innate defense system of humans. Patients who suffer urinary tract infection (UTI) will generate LL-37 and which is released into urine. LL-37 can be used as an indicator for the diagnosis of UTI. We have designed a biosensor with an interdigitated electrode on a printed-circuit board (PCB). The surface of the electrode was modified with 3-mercaptopropionic acid and immobilized with anti-LL37 antibody to improve the specificity of the biosensor. By de-embedding jig impedance, the impedance associated with the change of LL-37 concentration was calculated. The sensitivity of this biosensor for LL-37 in a urine sample can reach 50 μg/mL.
Keywords :
Biosensors; Capacitance; Electrodes; Impedance; Molecular biophysics; Peptides; Surface impedance; Biosensor; LL-37; impedance; interdigitated electrode; urinary tract infection; Antimicrobial Cationic Peptides; Biosensing Techniques; Conductometry; Electric Impedance; Electrodes; Equipment Design; Equipment Failure Analysis; Humans; Immunoassay; Reproducibility of Results; Sensitivity and Specificity;
Conference_Titel :
Engineering in Medicine and Biology Society, EMBC, 2011 Annual International Conference of the IEEE
Conference_Location :
Boston, MA
Print_ISBN :
978-1-4244-4121-1
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2011.6089899
