Title :
Measurement of organ blood flow with coloured microspheres: a first time-saving improvement using automated spectrophotometry
Author :
Wieland, W. ; Wouters, PF ; Van Aken, H. ; Flameng, W.
Author_Institution :
Centre for Exp. Surgery & Anaesthesiology, Katholieke Univ., Leuven, Belgium
Abstract :
The authors report on the development and application of automated spectrophotometry for regional blood flow measurement using the coloured microsphere (CM) dye-extraction technique. A software program running under Microsoft Windows 3.1 was written to simultaneously control an autosampler, sipper pump, pressure valves and spectrophotometer with an exclusively designed small-volume flow-cell. The system allowed consecutive measurements of over 200 tissue samples in volumes as small as 40 μL without any attendance. The system was validated in eight chronically instrumented dogs. A comparison between the advanced CM technique and the standard radionuclide microspheres method demonstrated a strong correlation for blood flows to various organs in awake animals. The system proved to be a reliable, user-friendly and time-saving application for the CM dye-extraction technique
Keywords :
biological techniques; biology computing; blood flow measurement; spectrophotometry; Microsoft Windows 3.1; automated spectrophotometry; awake animals; biological research technique; chronically instrumented dogs; coloured microspheres; organ blood flow measurement; pressure valves; sipper pump; small-volume flow-cell; software program; standard radionuclide microspheres method; tissue samples; Animal structures; Application software; Automatic control; Blood flow; Dogs; Fluid flow measurement; Instruments; Pressure control; Valves; Volume measurement;
Conference_Titel :
Computers in Cardiology 1993, Proceedings.
Conference_Location :
London
Print_ISBN :
0-8186-5470-8
DOI :
10.1109/CIC.1993.378308
